The FIT Test 2018-02-01T10:05:49+00:00

The FIT Test

The FIT Tests offered by KBMO Diagnostics were designed to measure both IgG and immune complexes simultaneously which is why the tests are more sensitive than others which measure only IgG.

The FIT Tests offer testing on up to 132 common foods designed to help a person identify problem foods so they can be removed from the diet. When these problem foods are eliminated from the diet, many times the symptoms associated with food sensitivities can be alleviated.

The FIT Tests: A More Detailed Description

The serum sample is diluted, added to each well of an ELISA plate which is coated with food antigens. During incubation, antibodies from the sample bind to the food antigens. The plate is washed to remove unbound antibody followed by addition of a conjugate containing both anti-Human-IgG and anti-Human-Complement-C3d-HRP. The conjugates bind to HuIgG antibodies and to C3d which is attached to the IgG contained in the Immune Complex lattice (IC-C3d). The plate is washed to remove the unbound conjugate and substrate is added to visualize the bound HuIgG and IC-C3d. A unique program analyzes the data and generates a report that is easy to understand. The foods that have a bar which extends above the baseline are considered positive and should be eliminated from the diet. Elimination of the offending foods from the diet should help reduce the symptoms associated with a particular condition after a few weeks.

In addition we also offer custom food sensitivity panels to suit our customer’s needs that utilize the same dual measurement technology.

The Fit Test

The merits of using the FIT Tests to detect Food Sensitivities

The FIT Tests: Comparison with conventional IgG only tests:

Food sensitivity tests that measure IgG alone are less sensitive than the FIT Tests which measure both IgG and Immune Complexes. The net result is that tests measuring only IgG will under report the occurrence of foods that cause food sensitivities. The FIT Tests which measure both IgG and Immune Complexes is more sensitive and generates results that give a 95% confidence that foods are either positive or negative in an easy to interpret and understand report whereas, IgG only tests that give broad ranges of reactivity which are hard to interpret and even harder for the patient to understand.

The FIT Tests employ a conjugate which contains bothanti-Human IgG and anti-Human-C3d linked to an enzyme. The anti-Human-IgG is specific for IgG heavy chains subclasses 1-4 whereas, the anti-C3d reacts with an epitope on C3d which is covalently bound to IgG in the immune complex (IC-C3d). The dual conjugate binds to both IgG and C3d and increases sensitivity when compared to conventional tests that use only anti-Human IgG. The increased sensitivity of the FIT Tests over conventional IgG only tests are illustrated by the data in the patent (#8,309,318)

The FIT Test: Practical implications

The information gained from the FIT Tests can be directly applied to diet management with the overall goal of lessening the symptoms associated with certain conditions. If the foods that screen positive for immune complexes and IgG are eliminated from the diet, inflammation should be reduced and the patient should experience some relief from the symptoms associated with certain conditions. Our tests are not a “cure all” solution but rather a tool which identifies potentially harmful foods which then can be eliminated from the diet in an attempt to lessen symptoms associated with conditions that are refractory to conventional techniques. Unlike medicines that have side effects, diet management is a natural process that controls the intake of reactive foods, while at the same time, providing complete nutrition to prevent any undesired side effects.